© Benaki Phytopathological Institute
Resistance of medicinal plants to root-knot nematodes
7
al.,
1984) and selection of resistant sources
of different plants to root-knot nematodes
(Talwana
et al.,
1997; Cervantes-Flores
et al.,
2008; Marchese
et al.,
2010; Mudiope
et al.,
2012; Gomes
et al.,
2015; Karuri
et al.,
2017).
Materials and Methods
Preparation of nematode inoculum
Eggs of
M. javanica
were extracted from
galled root of tomato (
Solanum lycopersi-
cum
cv. Early-Urbana) using the sodium hy-
poclorite method (NaOCl) (Hussey and Bark-
er, 1973). Infected roots collected from the
greenhouse of Boyer-Ahmad region were
chopped to 2-3 cm pieces and were shak-
en in the 0.5 % sodium hypochlorite (NaO-
Cl) for 90 seconds and poured into a stack of
two sieves, with a 75 μm aperture size at top
followed by a 25 μm aperture size. Eggs re-
tained on 25 μm aperture size sieve, which
were washed quickly to remove all NaO-
Cl and were counted under a stereomicro-
scope.
Pot experiments
The experiments were conducted under
greenhouse conditions at 28 ± 4ºC under 16:8
h (light : dark) photoperiod. Seeds of
Physalis
alkekengi
L. (alkakengy) (Solanaceae),
Matri-
caria chamomilla
L. (chamomile) (Asteraceae),
Rumex acetosella
L. (sorrel) (Polygonaceae),
Plantago lanceolata
L. (English plantain) (Plan-
taginaceae),
Foeniculum vulgare
Mill. (fennel)
(Apiaceae),
Anchusa italica
Retz. (garden an-
chusa) (Boraginaceae),
Marrubium vulgare
L.
(horehound) (Lamiaceae),
Levisticum officina-
Table 1
. Degree of resistance (DR) of medicinal plants to the root-knot nematodes
Meloidog-
yne javanica
and
Meloidogyne incognita
(Walker, 1995; Baida
et al
., 2011).
Medicinal plants
DR to
M. incognita
race 3
(Walker, 1995)
DR to
M. incognita
(Baida
et al
., 2011)
DR to
M. javanica
(Baida
et al
., 2011)
Anethum graveolens
L.
Artemisia absinthium
L.
Erurca vesicaria
L.
Foeniculum vulgare
Mill.
Hyssopus officinalis
L.
Lavandula augustifolia
Mill.
Nepeta cataria
L
Ocimum basilicum
L.
Salvia officinalis
L.
Thymus vulgaris
L.
Mikania glomerata
Sprengel.
Pimpinella anisum
L.
Coriandrum sativium
L.
Matricaria recutita
L.
Melissa officinalis
L.
Mentha piperita
L.
Origanummajorana
L.
Origanum vulgare
L.
Rosmarinus officinalis
L.
Ruta graveolens
L.
Satureja hortensis
L.
Tanacetum vulgare
L
.
Tomato (control)
Mentha pulegium
L.
Plectranthus barbatus
Andr.
Commiphora myrrha
(Nees) Engl.
Carpobrotus edulis
(
L.
)
N.E. Br
Plectranthus neochilus
Schltr.
H
H
H
H
H
H
H
H
H
H
-
-
S
S
S
-
S
S
S
S
S
S
-
-
-
-
-
-
H
-
-
-
H
-
-
H
-
H
H
R
-
S
-
-
R
-
-
R
-
-
S
R
R
R
R
R
H
-
-
-
H
-
-
H
-
H
H
H
-
S
-
-
R
-
-
R
-
-
S
R
R
R
R
R
S = Susceptible; H = Hypersusceptible; R=Resistance
